Regulatory
Part:BBa_K080000
Designed by: Jennifer Lei Group: iGEM08_Bay_Area_RSI (2008-10-20)
TCFbs-BMP4
Induction of BMP4 during early stages of cardiomyocyte differentiation results in inhibitory effects. From previous studies it is not clear whether BMP4 is necessary for cardiomyocyte differentiation. In our circuit, nkx2.5 and gata4 will be induced and initiate the pathways of cardiac differentiation. During normal cardiac muscle differentiation BMP4 is a downstream target of nkx-2.5 and gata4, the expression of which turns on at later stages of cardiac differentiation. To ensure that BMP4 has a delayed expression, we designed BMP4 under the control of minimal promoters of late markers of cardiac muscle differentiation, TCF and alpha cardiac actin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 13
Illegal SpeI site found at 946
Illegal PstI site found at 293
Illegal PstI site found at 993 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1565
Illegal SpeI site found at 946
Illegal PstI site found at 293
Illegal PstI site found at 993 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 13
Illegal SpeI site found at 946
Illegal PstI site found at 293
Illegal PstI site found at 993 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 13
Illegal SpeI site found at 946
Illegal PstI site found at 293
Illegal PstI site found at 993 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 419
Illegal SapI.rc site found at 623
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Categories
Parameters
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